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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells

Fig. 1

Design and validation of the piggyBac-TANGO assays. a Schematic overview of the expression vectors used throughout this study. b Scheme of generation of stable cell lines expressing piggyBac-TANGO. c Validation of selected receptors in the piggyBac-TANGO assay. d Fluorescence intensity of DRD2 stimulated by cabergoline in the piggyBac-TANGO assay. e Concentration-response curves of DRD2 stimulated by cabergoline in the cAMP assay. Data are presented as the means ± SEM (n = 3). Caber: Cabergoline. Curves were fitted using GraphPad Prism 6

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