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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: DRAM1 regulates autophagy and cell proliferation via inhibition of the phosphoinositide 3-kinase-Akt-mTOR-ribosomal protein S6 pathway

Fig. 7

DRAM1 inhibits rpS6 phosphorylation in human cancer cells. a, b and c HEK293T, SW480 and HCT116 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h. The protein levels of p-rpS6 (S235/236, S240/244), rpS6, FLAG and β-actin were detected with immunoblotting. d and e Quantitative analysis of the optical densities of p-rpS6 (S235/236, S240/244) and rpS6. Data represent mean ± SEM of combined data from three independent experiments. *p < 0.05 and **p < 0.01 vs the indicated groups

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Cell Communication and Signaling

ISSN: 1478-811X