Fig. 7
DisBa-01 induces morphological changes in endothelial cells. HUVECs (3 × 104 cells/well) were plated in a 96-well microplate previously coated with FN (1 μg/cm2), in serum-free DMEM and incubated overnight at 37 °C, 5% CO2. Cells were exposed to VEGF (10 ng/mL), DisBa-01 (1000 nM) and VEGF plus DisBa-01 for 30 min in DMEM 10% FBS. Cell nuclei were stained with DAPI (0.7 ng/μl) and cytoplasm was stained with Alexa Fluor™ 488 phalloidin for 10 min. Images were observed with 60x magnification. Representative images were obtained from three independent experiments. Scale bar = 50 μm (left panel) and 20 μm (right panel)