Fig. 6

DisBa-01 promotes FAK, Src and paxillin phosphorylation. HUVECs (5 × 10⁵ cells/well) were seeded in 6-well plates and left to adhere at 37 °C, 5% CO₂, overnight, followed by a period of 24 h of starvation at serum-free medium. Cells were treated with 1 ml of DMEM supplemented with 10% FBS and either DisBa-01 (1000 nM), VEGF (10 ng/mL) or a co-treatment and incubated for 1 and 24 h at 37 °C, 5% CO₂, followed by cell lysis. Twenty micrograms of protein from the cell lysate were separated on SDS-PAGE. Blots were probed with antibodies to a P-Y397 FAK and anti-FAK; to b P-TY418 Src; to c phospho LIM1 Paxilin; and GAPDH, this last used to normalize loading. Bands corresponding to all proteins were quantified by densitometry using the ImageJ FIJI program. Bar graph shows the mean ± SE of phosphorylated pFAK/FAK/GAPDH, pSrc/GAPDH and pLIM1Paxilin/GAPDH expression from three independent experiments. Values of *p < 0.05 were significantly different when compared to untreated (a), DisBa-01 (b) and VEGF (c) groups