Fig. 2

DisBa-01 inhibits HUVEC tubulogenesis. HUVECs (3 × 10⁴ cells/well) were treated for 30 min with VEGF (10 ng/mL), DisBa-01 (1, 10, 100 and 1000 nM) or VEGF plus DisBa-01 (1000 nM) in DMEM containing 0.5% FBS and then seeded on a solidified Matrigel. The plate was placed in a humidified CO₂ incubator at 37 °C for 14 h to allow the formation of tubes. a Photos (40x magnification) were obtained from a representative experiment (n = 3). The results were expressed as b Total length (μm²), c Number of mashes, d Number of nodes, e Number of master junctions and f Angiogenesis Score (analysed area x tube length x total of branches). Images were photographed using the AxionVision Rel.4.8 software of a Vert.A1 microscope (Zeiss) and analysed using the Angiogenesis Analyzer plugin for ImageJ software (version 1.51n). Results represent the average of three independent experiments in triplicate. Values of *p < 0.05 were significantly different when compared to untreated (a), DisBa-01 (b) and VEGF (c) groups