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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Flavokawain B targets protein neddylation for enhancing the anti-prostate cancer effect of Bortezomib via Skp2 degradation

Fig. 1

FKB inhibits the neddylation of Cullin1 and Ubc12. a, PC3 cells were treated with indicated concentrations of FKB for different periods of time. Expression of NEDD8 and its modified proteins were measured by Western blotting analysis. b, Western blotting analysis of Cullin1 neddylation in vehicle control (0.1% DMSO) or 8.8 μM FKB treated LNCaP and PC3 cells. c, Cullin1 neddylation was measured via immunoprecipitation with anti-Cullin1 and then Western blotting analysis of the immunoprecipitates by anti-NEDD8 antibody in vehicle control or 8.8 μM FKB treated DU145 and PC3 cells. d, PC3 cells were treated with 8.8 μM FKB or 1 μM MLN4924 for 16 h. Neddylation of Cullin1 and Ubc12 and expression of ubiquitinated proteins and Skp2 were examined by specific antibodies and β-tubulin serves as a loading control

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Cell Communication and Signaling

ISSN: 1478-811X