Fig. 5
From: Autocrine motility factor promotes endometrial cancer progression by targeting GPER-1
AMF expression correlated with GPER-1 expression in clinical samples. a. Immunohistochemical analysis of AMF and GPER-1 expression in the normal endometrium (n = 50) and EC, including type I EC (n = 52) and type II EC (n = 47). b. Statistical summary of the immunostaining intensities of AMF and GPER-1 in tissues; AMF and GPER-1 are highly expressed in endometrial cancer (***P < 0.001), and their expression levels are positively correlated (r = 0.92, P < 0.01). c. AMF and GPER-1 expression in EC tissue specimens and normal endometria was assessed by qRT-PCR and normalized to β-actin expression (**P < 0.01, ***P < 0.001, unpaired Student’s t-test). d. The interaction between AMF and GPER-1 was detected by coimmunoprecipitation, and each group included three cases. e. Immunofluorescence was used to confirm the AMF-GPER-1 colocalization in clinical specimens. f. Kaplan–Meier analysis of TCGA uterine corpus endometrial carcinoma showing the correlation between PGI expression and relapse-free survival. g. Kaplan–Meier analysis showing the correlation between GPER-1 expression and relapse-free survival in PGI high expressed samples. (P-value calculated by log-rank test)