Fig. 4
From: Autocrine motility factor promotes endometrial cancer progression by targeting GPER-1
The effects of silencing GPER-1 on AMF-induced tumorigenicity in vivo. Under the indicated conditions, mock or shGPER-1 cells with AMFR deleption, containing luciferase were injected (6 × 105 cells per mouse) with or without exogenous AMF, and 8-week-old nu/nu female mice were randomly allotted to four groups (n = 10 mice per group). a. Tumor metastasis over a 6-week period by bioluminescence analysis. b. Quantitative analysis of metastatic cells based on bioluminescence imaging. The means and 95% confidence intervals (error bars) are presented; ***P < 0.001. P values were calculated using a two-sided Student’s t-test. p/sec/cm2/steradian. c. Macroscopic view of intraperitoneal injection-derived tumor metastasis. Black arrow, tumor metastasis. d. Tumor metastasis per mouse was calculated and measured. e. Average volumes of tumor metastasis in the four groups. P values were calculated using two-sided Student’s t-tests; ***P < 0.001; NS, not significant. f. Kaplan–Meier analysis of mouse survival after xenograft implantation. P values were calculated using two-sided log-rank tests (*P < 0.05, NS, not significant). g. Representative H&E histopathology analyses of ovarian metastases in mice; AMF, GPER-1, Ki-67 and p-AKT expression was detected by immunohistochemistry (magnification, 200×)