Fig. 2

1,25(OH)₂D₃ regulates IFNγ and IL-1β expressions dependent on HIF-1α pathway. a and b Western blot measurements of HOKs treated with LPS (100 ng/ml) or saline in the presence or absence of 20 nM 1,25(OH)₂D₃ (a), and corresponding densitometric analysis (b). c and d Real-time PCR quantification (c) and western blot analyses (d) of scramble siRNA-transfected HOKs followed by different treatments as shown. e and f Real-time PCR quantification (e) and western blot analyses (f) of hHIF-1α-siRNA-transfected HOKs followed by various stimulations as indicated. g and h Western blot measurements of HOKs transfected with HIF-1α plasmids or control vector with or without 20 nM 1,25(OH)₂D₃ pre-treatment (g), and densitometric analysis (h). *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding controls; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS or HIF-1α; Ctrl, control; 1,25VD, 1,25(OH)2D3. All assays were conducted for three times, n = 3