Fig. 5
From: The balance of Bmp6 and Wnt10b regulates the telogen-anagen transition of hair follicles
BrdU labeling in KRT15 positive HFSCs. Krt15 and BrdU were detected by immunofluorescence at 1 day after treatment (a, b), 2 days after treatment (c, d), 3 days after treatment (e-h) and 7 days after treatment (i, j). a-f, i, j BrdU was injected at 4 h before sampling. g, h BrdU was injected at the time of adenovirus treating. Green color shows the positive expression of Krt15. Red color shows the BrdU positive cells. DAPI was used to counterstain the nucleus. a, c, e, g, i The right panels are the merged picture of protein markers and DAPI. Scale bar = 10 μm. b, d, f, h, j are the bar charts of the ratio of BrdU positive cells in Krt15 positive cells. HF, hair follicle. N = 6, * P < 0.05