Fig. 5

Lovastatin synergizes with lapatinib to suppress HCC1954 xenograft growth. a HCC1954 cells were inoculated into female nude mice to generate xenograft mouse models. Mice were randomized into 3 groups to receive control vehicle, lapatinib, or the combination of lovastatin and lapatinib as described in the methods section. The sizes of xenografts were measured every 3 days and calculated tumor volumes were plotted. Presented graph shows data from 5 mice per group. Error bars show the standard error of the mean (SEM), with ** indicating p < 0.01. b after 17 days of oral gavage treatment, the mice were sacrificed and xenograft tumors were resected. c tumor weight was measured and plotted. Presented graph shows data from 5 mice per group. Error bars show the standard error of the mean (SEM), with * and ** indicating p < 0.05 and p < 0.01, respectively. d protein samples were prepared from xenograft tumor tissues (3 per treatment group) and analyzed by immunoblotting using indicated antibodies. Vinculin was probed to show equal loading. Bar chart below shows the relative quantification of ErbB2