Fig. 4

Lovastatin addition results in enhanced internalization and degradation of ErbB2. a SKBR3, AU565, and HCC1954 cells were treated with lovastatin (40 μM), lapatinib (200 nM), neratinib (200 nM), or combinations as indicated for 24 h. Cell lysates were analyzed by immunoblotting with indicated antibodies. Column graphs below show the quantification of relative ErbB2 abundance compared to control samples. All error bars represent the standard error of the mean (n = 3), with * and ** indicating p < 0.05 and p < 0.01, respectively. b HCC1954 cells were treated with lapatinib (200 nM) or neratinib (200 nM) before immunofluorescence analysis to inspect ErbB2 staining. DAPI stains the nucleus. Scale bar = 10 μm. Images were captured using a fluorescent microscope (Olympus BX63, 40X objective). c cultured HCC1954 cells were treated with lovastatin (40 μM) either alone or in the presence of lapatinib or neratinib (both at 200 nM). Immunofluorescence assays were then carried out as in b