Fig. 6

Oroxylin A inhibition of LDH-A led to reduction of HSC contraction. LX2 cells were treated with oroxylin A and/or galloflavin at indicated concentrations, or transfected with LDH-A siRNA for 24 h. a Collagen gel contraction assays with quantification. b Cytoskeleton fluorescence staining, scale bar: 20 μm. c Western blot analyses of MLC2 phosphorylation with quantification. d Immunofluorescence analyses of MLC2 phosphorylation, scale bar: 20 μm. e Cytoskeleton fluorescence staining, scale bar: 20 μm. f Western blot analyses of MLC2 phosphorylation with quantification. For statistical significance of panels a-d: *p < 0.05 vs. control, **p < 0.01 vs. control. For statistical significance of panels e-f: **p < 0.01 vs. control siRNA