Fig. 4

Hp-mediated pAbl^T735 leads to cytoplasmic localization. AGS cells were transfected with Abl^wt (a) or Abl^TA (b). Cells were left uninfected (mock) or infected with Hp for 4 h. Abl (green) and phalloidin (red) were merged with DAPI staining (blue). White arrows indicate perinuclear and cytoplasmic staining of c-Abl. Bar, 20 μm. (c) The lengths of approximately 100 transfected AGS cells expressing Abl^wt or Abl^TA were determined after infection with Hp for 4 h. (d) Stable AGS TAP-Abl^wt and TAP-Abl^TA cells were grown in transwell filters and left untreated (white bars) or infected with Hp (black bars) for 24 h