Fig. 1From: The protein kinase Cmk2 negatively regulates the calcium/calcineurin signalling pathway and expression of calcium pump genes PMR1 and PMC1 in budding yeastPhenotypes of calcium-tolerant gene deletion mutants. Cells of the wild-type BY4743 and five deletion mutants identified from the library screen were grown at 30 °C in liquid YPD overnight, serially diluted by 10 times and spotted on YPD plates with or without supplemented CaCl2 or cyclosporine A (CsA) as indicated, respectively. Plates were incubated for 2 to 3 days at 30 °CBack to article page