Fig. 4

Memantine co-treatment results in increased CytC release and activation of Caspase-9 and Caspase-3. a Jurkat cells were cultured with memantine and AraC±memantine as indicated. After 72 h intracellular CytC level was determined with flow cytometry. CytC^low cells represent apoptotic cells having released mitochondrial CytC. Data of n = 5 independent experiments is shown as mean ± SD. b Expression of Caspase-9 and the cleaved active form of Caspase-3 in Jurkat cells treated without and with drug as indicated was analyzed by Western blot. The data in bar graphs give the mean + SD relative expression of each indicated protein calculated from densitometric quantifications of n = 4 independent experiments. c Cell death of F9 and JMR cells cultured without or with AraC±memantine for 72 h was determined with PI staining. The percentage of PI⁺ cells of n = 5 independent experiments is shown as mean ± SD. Western blot shows Caspase-9 and ß-Actin expression in F9 and JMR cells