Fig. 3

Effects of Cori treatment on Keap1-Nrf2/ARE signaling pathway in HepG2 cells. a HepG2 cells were exposed to concentration of Cori (30 μM) for three time points (1, 3 or 6 h), and the total protein were determined by Western blotting analysis. b Keap1-siRNA or control siRNA were transfected into cells for 24 h and were collected; proteins were detected by Western blotting analysis. c Keap1 mediates Cori-induced Nrf2 protein expression. Keap1-siRNA or control siRNA were transfected into cells for 24 h, then were treated with Cori (30 μM) for 6 h. d-e Cells were subjected to Cori (30 μM) for three time points (1, 3 or 6 h) or Cori (7.5, 15, or 30 μM) for 6 h, and the nuclear and cytoplasmic levels of Nrf2 were examined by Western blotting analysis. The relative density of protein was performed by densitometric analysis; β-actin and Lamin B were acted as an internal control. In addition, (f-g) the luciferase plasmids pGL-ARE and pRL-TK was transiently transfected into cells for 24 h and subsequently exposed to 30 μM Cori for (1, 3 or 6 h) or Cori (7.5, 15, or 30 μM) for 6 h. ARE luciferase activity was detected by a dual-luciferase reporter assay system. Similar results were obtained from three independent experiments. All results were expressed as means ± SEM of three independent experiments. ^#p < 0.05 and ^##p < 0.01 vs the Control group