Fig. 1
From: Heme oxygenase 1 facilitates cell proliferation via the B-Raf-ERK signaling pathway in melanoma
B-Raf is related to HO-1 in melanoma. a Hematoxylin and eosin (HE) and immunohistochemical staining of HO-1 and B-Raf were performed on paraffin-embedded sections from adjacent healthy tissues (upper panel) and melanoma tissues (lower panel). Scale bar: 100 μm. b The protein levels of B-Raf were measured in A375 cells with HO-1 overexpression or knockdown. c The Flag-B-Raf expression plasmid was co-transfected with or without HA-HO-1 into HEK293T cells. HO-1 protein was immunoprecipitated with anti-HA antibody and immunoblotted with antibodies against HA and Flag. d Colocalization of B-Raf and HO-1 in A375 cells co-transfected with HO-1 and BRAF expression plasmid. Scale bar: 10 μm