Fig. 3
From: Blockade of ARHGAP11A reverses malignant progress via inactivating Rac1B in hepatocellular carcinoma
ARHGAP11A facilitates HCC cell invasion, migration and EMT. Columns, mean (n = 3, in triplicate); bars, SD. *, P < 0.05 versus NC. a Ingenuity Pathway Analysis (IPA) showed the functional pathways that were downregulated in Hep3B cells upon ARHGAP11A suppression. The orange line represents the cutoff value for significance. b Invasion ability of ARHGAP11A- knockdown HCC cells. Representative photos of the Transwell assay are shown. c Migration ability of ARHGAP11A-knockdown HCC cells. Representative photos of the wound healing assay are shown. d Representative microscopic images captured with inverted fluorescence microscope (OLYMPUS-DP72, Japan) are shown for the morphological changes of ARHGAP11A-knockdown HCC cells. Arrowheads, spindle-shaped cells. Scale bars, 50 μm. e E-cadherin, N-cadherin, Snail and Slug expression in ARHGAP11A-knockdown HCC cells was measured by western blot