Fig. 6

Adipocyte-derived IL-6 and leptin activate the JAK/STAT3 and AKT signaling pathways to promote PLOD2 expression. a MDA-MB-231 (MB-231) cells were monocultured or cocultured with adipocytes. An inhibitor directed against janus kinase (ruxolitinib) or PBS was added to the culture medium. After 3 days, tumor cells were harvested to detect protein expression levels of PLOD2, P-STAT3 and STAT3. b MDA-MB-231 (MB-231) cells were grown on coverslips in inserts. Cells in the lower chamber were monocultured or cocultured with adipocytes and treated with either ruxolitinib or PBS. After 3 days, cell were fixed and stained for P-STAT3. Nuclei were stained with Hoechst. Scale bars, 20 μm. c MDA-MB-231 (MB-231) cells were monocultured or cocultured with adipocytes. Cells were treated with either an inhibitor directed against phosphatidylinositol 3-kinase (LY294002) or PBS. After 3 days, tumor cells were harvested to detect protein expression levels of PLOD2, P-AKT and AKT. d MDA-MB-231 (MB-231) cells were monocultured or cocultured with adipocytes. After 3 days, tumor cells were harvested to detect protein expression levels of PLOD2, P-ERK1/2 and ERK1/2. e MDA-MB-231 (MB-231) cells were treated with or without IL-6 plus either ruxolitinib or PBS. After 12 h, tumor cells were harvested to detect protein expression levels of PLOD2, P-STAT3 and STAT3. f MDA-MB-231 (MB-231) cells were grown on coverslips and treated with or without IL-6. Ruxolitinib or PBS was added to the culture medium. After 12 h, cells were fixed and stained for P-STAT3. Nuclei were stained with Hoechst. Scale bars, 20 μm. g MDA-MB-231 (MB-231) cells were treated with or without leptin. Ruxolitinib or PBS was added to the culture medium. After 3 days, tumor cells were harvested to detect protein expression levels of PLOD2, P-STAT3 and STAT3. h MDA-MB-231 (MB-231) cells were grown on coverslips and treated with or without leptin. Ruxolitinib or PBS was added to the culture medium. After 3 days, tumors cells were fixed and stained for P-STAT3. Nuclei were stained with Hoechst. Scale bars, 20 μm. i MDA-MB-231 (MB-231) cells were treated with or without leptin. LY294002 or PBS was added to the culture medium. After 3 days, tumor cells were harvested to detect protein expression levels of PLOD2, P-AKT and AKT