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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Tyro3, Axl, and Mertk receptors differentially participate in platelet activation and thrombus formation

Fig. 5

GPVI-stimulated protein tyrosine phosphorylation is inhibited in the absence of Tyro3 and Axl, but not Mertk. After wild-type and Tyro3−/− platelets were stimulated with 30 ng mL− 1 convulxin for the indicated time period, platelets were solubilized and the lysates were subjected to immunoprecipitation with antibody against PLCγ2. Whole cell lysates (a) or anti-PLCγ2 immunoprecipitates (b) were analyzed for tyrosine phosphorylation by immunoblotting using PY99. Equal protein loading was assured by probing the samples with antibody against PLCγ2. (c) Tyro3−/−, Axl−/−, Mertk−/−, and wild-type platelets were stimulated with 30 ng mL− 1 convulxin for 2 min, platelets were solubilized and the lysates were subjected to immunoprecipitation with antibody against Syk. The anti-Syk immunoprecipitates were analyzed for tyrosine phosphorylation using PY99. The blots are representative of three independent experiments

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