Fig. 5

GPVI-stimulated protein tyrosine phosphorylation is inhibited in the absence of Tyro3 and Axl, but not Mertk. After wild-type and Tyro3^−/− platelets were stimulated with 30 ng mL^− 1 convulxin for the indicated time period, platelets were solubilized and the lysates were subjected to immunoprecipitation with antibody against PLCγ2. Whole cell lysates (a) or anti-PLCγ2 immunoprecipitates (b) were analyzed for tyrosine phosphorylation by immunoblotting using PY99. Equal protein loading was assured by probing the samples with antibody against PLCγ2. (c) Tyro3^−/−, Axl^−/−, Mertk^−/−, and wild-type platelets were stimulated with 30 ng mL^− 1 convulxin for 2 min, platelets were solubilized and the lysates were subjected to immunoprecipitation with antibody against Syk. The anti-Syk immunoprecipitates were analyzed for tyrosine phosphorylation using PY99. The blots are representative of three independent experiments