Fig. 1

Amplification of IMPACT drives its increased expression in a wide range of human malignancies. a) IMPACT expression in tumours and non-cancer tissues. The middle line inside each box represents median, the left and right hinge denote lower and upper quartile, respectively, and whiskers extend to 1.5x interquartile range. Open circles denote outliers; and for clarity, 112 outliers have been removed. b) Differential expression of IMPACT between cancer and non-cancer tissues. Each bar indicates a fold change difference (log2 FC) between a log₂ transformed median mRNA count in each cancer type and pooled non-cancer samples within each organ classification. c) Boxplot of methylation (mean β-value) of the IMPACT promoter site. β-values < 0.2 indicate hypo-methylation. d) Boxplot of the estimated copy number of IMPACT. The value of 1 represents a diploid copy number. The middle vertical line, and left and right hinges in the boxes in Fig. 1c and d indicate median, 25th and 75th percentile, respectively. e) Absolute values of Spearman’s rank correlations (grey scale gradient) between the expression of IMPACT and IMPACT copy number variation (CNV) or methylation of the IMPACT promoter (450 K). Larger circle area indicates statistically significant correlations (P ≤ 0.05) and vice versa. Tabulated values for Fig. 1e and additional details are available in Additional file 3