Fig. 6
From: Regulation of MRTF-A by JMY via a nucleation-independent mechanism
Recombinant V/VVVCA domains of JMY compete with actin for MRTF-A binding in vitro. Association of MRTF-A-f.l.-HA with Flag-actin-WT precipitates was investigated in the presence of cleared extracts of GST/-JMY-V/VVVCA-expressing Escherichia coli BL21 (DE3) Rosetta cells. Immuno-precipitates (upper panel) and lysate controls (lower panel) were analyzed by western blotting with the depicted primary antibodies. Asterisk indicates unspecific cross-reaction of GST antibody with E. coli proteins