Table 1 List of forward and reverse primers used for qPCR analysis
From: SOX2 as a novel contributor of oxidative metabolism in melanoma cells
Gene | Forward | Reverse |
|---|---|---|
GLUT1 | CGGGCCAAGAGTGTGCTAA | TGACGATACCGGAGCCAATG |
GLUT3 | CGAACTTCCTAGTCGGATTG | AGGAGGCACGACTTAGACAT |
HK2 | CAAAGTGACAGTGGGTGTGG | GCCAGGTCCTTCACTGTCTC |
LDHA | AGGGAATGTACGGCATTGAG | CCTCATCGTCCTTCAGCTTC |
PDK1 | CCAAGACCTCGTGTTGAGACC | AATACACGTCTCAGGTCTCCTTGG |
PDP2 | TAGGCCAACCTTTGTTTCACCA | AGACCCTCACAACAAAAGCCT |
MCT-1 | GTGGCTCAGCTCCGTATTGT | GAGCCGACCTAAAAGTGGTG |
MCT-4 | CAGTTCGAGGTGCTCATGG | ATGTAGAGGTGGGTCGCATC |
PGC1α | GGGAAAGTGAGCGATTAGTTGAG | CATGTAGAATTGGCAGGTGGAA |
CytC | TTGCACTTACACCGGTACTTAAGC | ACGTCCCCACTCTCTAAGTCCAA |
COX4I | GGCCCGGCATTTTACGA | TCACCGTGGAGCGGAAA |
COX5b | TGCGCTCCATGGCATCA | CCCAGTCGCCTGCTCTTC |
ATP5A1 | TGCAAGGACTTCCATGCCTC | CGCCCAGGTTCTTCAAGATCAA |
SOX2 | GAGCTTTGCAGGAAGTTTGC | GCAAGAAGCCTCTCCTTGAA |
HIF1a promoter | TGCAAAGTTGCCAAAGGCCA | CAGGGGAACTCACCTTGTCTAC |
HIF1a | GGCGCGAACGACAAGAAAAA | TCCAAATCACCAGCATCCAGA |
TBP | CAACAGCCTGCCACCTTAC | CTGAATAGGCTGTGGGGTC |
ACTIN | TCGAGCCATAAAAGGCAACT | CTTCCTCAATCTCGCTCTCG |
GAPDH | GACGCTGGGGCTGGCATTG | GCTGGTGGTCCAGGGGTC |
Β2-microglobulin | GCCGTGTGAACCATGTGACT | GCTTACATGTCTCGATCCCACTT |