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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Akt1 inhibition promotes breast cancer metastasis through EGFR-mediated β-catenin nuclear accumulation

Fig. 3

Knockdown of Akt1 could not promote the secretion of EGFR ligands and the mRNA expression of EGFR in MCF-7 and MDA-MB-231 cells. (a, b) The EGFR ligands EGF, HB-EGF, TGF-α, β-Cellulin and amphiregulin was detected in the cell culture medium using ELISA assay. Note that no significant alteration was observed on the secretion of EGF, HB-EGF, TGF-α, β-Cellulin and amphiregulin in the cells transfected with Akt1 siRNA, one-way ANOVA, n = 5 per group. (c) Real time PCR analysis showed that the expression of EGFR mRNA in MCF-7 and MDA-MB-231 cells after treated with Akt1 siRNA. Note that no significant alteration is observed on EGFR mRNA expression after treated with Akt1 siRNA for 24 h in MCF-7 and MDA-MB-231 cells, one-way ANOVA, n = 5 per group

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Cell Communication and Signaling

ISSN: 1478-811X