Fig. 1
From: Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide
Four-octyl itaconate activates Nrf2 signaling in neuronal cells. SH-SY5Y cells (a-d) or the primary murine neurons (e-h) were treated with applied concentration of 4-octyl itaconate (OI) for indicated time, mRNA expression of Nrf2-regulated genes and Ninj2 were tested by qPCR assay (a and e); Expression of listed proteins in total cellular lysates (b and f) and nuclear lysates (c and g) were tested by the Western blotting assays. Keap1-Nrf2 association was detected by co-immunoprecipitation assays (d and h). Expression of listed proteins were quantified and normalized to the loading control (b, c, f and g). Keap1-bound Nrf2 was quantified as well (d and h). Lamin-B1 was tested as a marker of nuclear protein (c and g). “Ctrl” stands for untreated control cells (Same for all Figures). Bars stand for mean ± standard deviation (S.D). * P < 0.05 vs. “Ctrl” cells