Fig. 8

P2X7-induced lysosomal rupture and cathepsin B activation contribute to cell death. a-c BV-2 cells were treated with A438079 (a and b), siAMPK (c) and/or BzATP for 6 h. Lysotracker Red staining was determined by flow cytometry. d After treatment with A438079, si-RNA against P2X7 or calcium-free medium, BV-2 cells were stimulated with BzATP for 6 h. Intracellular cathepsin B activity was determined using MagicRed cathepsin detection kit and fluorescence spectrophotometer. e BV-2 cells were treated with BzATP, either in the absence or presence of CA-074Me (10 μM) for the times indicated. TFEB localization in the cytosol and nuclei was determined. f After treatment with BzATP, either in the absence or presence of 3-MA (5 mM) and/or CA-074Me (10 μM) for 6 h, cell viability was determined by MTT assay. Data were the mean ± S.E.M. from 3 independent experiments. *p < 0.05, indicating the significant BzATP responses. #p < 0.05, indicating the significant effects of A438079, calcium free medium and CA-074Me  to antagonize or potentiate BzATP response. **p < 0.05, indicating the additive effect of 3-MA and CA-074Me on BzATP-induced cell death