Fig. 1
From: USP17 is required for trafficking and oncogenic signaling of mutant EGFR in NSCLC cells
(a-c) A549, HCC827, and H1975 cells were transfected as indicated and 72 h post-transfection USP17 mRNA expression was assessed by RT-PCR. B2M mRNA expression was also assessed as a loading control. (d-f) Equivalent numbers of A549, HCC827, and H1975 cells were transfected as indicated and 72 h post-transfection, the number of viable cells was determined by trypan-blue exclusion assay. (g-i) Equivalent numbers of A549, HCC827, and H1975 cells were transfected as indicated and 72 h post-transfection, cell viability was determined by MTT assay and the results reported as % of control. (j-l) A549, HCC827, and H1975 cells were transfected as indicated and 24 h post-transfection, equivalent numbers of cells were re-plated and subsequently incubated for a further 11 days. Colony numbers were then assessed and plotted as the survival fraction in comparison to control. Representative images are included in the bottom panels. ** p < 0.01, *** p < 0.001