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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Autologous blood transfusion augments impaired wound healing in diabetic mice by enhancing lncRNA H19 expression via the HIF-1α signaling pathway

Fig. 4

H19 promotes histone methylation by recruiting EZH2. a expression of relevant proteins binding to H19 in each group detected by western blot analysis after RNA-pull down assay; b expression of H19 combining with EZH2 in each group detected by RT-qPCR after RIP assay; c protein bands of H3K4me3 and GAPDH binding to EZH2 in each group determined by western blot analysis; d relative expression of H3K4me3 using western blot analysis; *, p < 0.05, compared with the normal group; The results were measurement data, which were presented as the mean ± standard deviation. Comparisons between two groups were analyzed by t-test. The experiment was repeated 3 times; normal, autologous blood preserved in standard preservative fluid; modified, autologous blood preserved in modified preservative fluid; RIP, RNA immunoprecipitation; RT-qPCR, reverse transcription quantitative polymerase chain reaction; EZH2, enhancer of zeste homolog 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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