Fig. 6

Insulin increased proliferation of INS-1 cells, but Nodal decreased proliferation. INS-1 cells were treated with culture medium alone or with 100 nM insulin,in the presence or absence of 1 μg/ml Nodal for 0–72 h. Cell proliferation was assessed by Brdu-Elisa. The proliferation was presented as percent of basal control absorbance values. In the histogram, the first column represents the basal control (=100%). n = 4. *, p < 0.05; **, p < 0.01, vs. control. ^▲, p < 0.05; ^▲▲, p < 0.01, vs. insulin. ^#, p < 0.05; ^##, p < 0.01, vs. Nodal