Fig. 8
MIF modulates MAPKs and PI3K/Akt phosphorylation in BLEL primary cells. (a) Influence of MIF on MAPKs and PI3K/Akt phosphorylation evaluated at 72 h and 1 week following treatment with MIF (200 ng/ml), ISO-1 (100 μM) or without MIF nor ISO-1. (b) Immunoblotting showing the kinetic of MIF-induced MAPKs and PI3K/Akt phosphorylation in BLEL primary cells. BLELp2 cells were seeded at a density of 2 × 105 cells/well in 24 well plate, let be adherent for 24 h and stimulated with MIF (200 ng/ml) at the indicated time point. Data were plotted as Mean ± SEM. * P-value < 0.05; ** P-value < 0.01; *** P-value < 0.001; no stars for P-value > 0.05