Fig. 3

Absence of Menin enhances wound-healing upon rapamycin treatment. Menin−/− and menin+/+ MEFs were subjected to a cell culture wound migration assay as described in Methods. The initial wound space was approximately 0.6 mm. After serum stimulation of cells, or in the presence of rapamycin or dual combination with wortmanin, the cell movement into the gap was imaged with a digital camera using a Zeiss microscope (a). The width of the open wound was measured with the T scratcher software. The data are presented as calculated percentage of the wound closure after 48 h relative to corresponding control (b). Only in menin+/+ MEFs rapamycin treatment significantly prevented wound healing and cell migration (P < 0.05). Data are presented as mean values (± SEM) in 5 independent experiments. Note that rapamycin significantly decreased wound healing in menin+/+ but not in menin−/− cells