Fig. 1
From: A role for RASSF1A in tunneling nanotube formation between cells through GEFH1/Rab11 pathway control
Bronchial as mesothelial cells establish both TNT1 and TNT2 and transfer mitochondria to other cells along TNT1. a–b Representative images of cytoskeletal elements presented in either TNT1 or TNT2. HBEC-3 and H28 cells were fixed and labelled with actin (green) and tubulin (red). Boxed regions were used for the zoom of each channel. c–d Structural analysis of TNTs subtypes in HBEC-3 and H28 cells through deconvoluted gCW STED nanoscopy. The cells were fixed and stained with Phalloidin for actin filaments. Arrowheads indicate the TNT2. e Representative image of TNT between HBEC-3 cells cultivated in 3D collagen matrix along with an optical sections reconstructed in three dimensions. f–g Representative image of TNT between H28 (f) and HBEC-3 (g) using scanning electron microscopy. h–i Montage of MitoTracker dye movement (arrow heads) along TNT in live HBEC-3 (h) and H28 (i) cells using time-lapse fluorescence video microscopy. Cells were imaged every min for 1 h. See Methods for protocol details