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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells

Fig. 3

Western blot analyses showing phosphorylation of a STAT3Tyr705, b AKTSer473 and c ERK1/2Thr202/Tyr204 in human vascular endothelial cells treated with IL-6 alone (50 ng/ml) or in combination with sIL-6R (100 ng/ml). One representative blot containing the phosphorylated protein, total protein and β-tubulin (loading control) is shown for each pathway (left column). The signals from the phosphorylated proteins and total proteins are first normalized to β-tubulin, and the ratio of the phosphorylated proteins and the total proteins are calculated. The graphs show arbitrary units (a.u., control is set to 1) compiled from 3 independent experiments presented as mean ± SEM for each pathway (right column). *p < 0.05, **p < 0.01, ***p < 0.001 compared to control

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