Fig. 2

The effect of RHBDD1 deletion on proliferation, migration and invasion in breast cancer cells. a CRISPR/Cas9-mediated RHBDD1-knockout system. MCF7 and MDA-MB-231 RHBDD1-knockout cells exhibited no RHBDD1 expression as determined by western blotting. GAPDH was a loading control. Experiments were repeated four times. b Cell proliferation assays. Each point represented the mean value of five independent samples. Experiments were repeated three times. c. and d. Representative photos and statistical plots of migration assays and Matrigel chemoinvasion assays. Original magnification, 200× (means±s.d., t test, ** p < 0.01; *** p < 0.001). Experiments were repeated three times