Fig. 4
FAF1 positively regulates JNK1 activity upon ischemic stress. a Upper panel: Faf1+/+ MEFs were transfected with the indicated concentrations of Flag-FAF1 plasmid. At 36 h after transfection, the cells were treated with oxygen glucose deprivation for 30 min. The cell lysates were subjected to immunoblot analysis with the indicated antibodies. Lower panel: the graph shows the results of the quantitative analysis of the P-JNK level (n = 3). b Upper panel: Faf1+/+ MEFs were transfected with VC or Flag-FAF1 plasmid (4 μg). At 36 h after transfection, the cells were treated with oxygen glucose deprivation for the indicated time periods. The cell lysates were immunoblotted with the indicated antibodies. Lower panel: the graph shows the results of the quantitative analysis of the P-JNK level (n = 3). c Upper panel: Faf1+/+ and Faf1gt/gt MEFs were treated with oxygen glucose deprivation for the indicated times, and then, cell lysates were immunoblotted with the indicated antibodies. Lower panel: the graph shows the results of the quantitative analysis of the P-JNK level (n = 3). d Upper panel: Faf1gt/gt MEFs were transfected with VC or Flag-FAF1 plasmid (4 μg). At 36 h after transfection, the cells were treated with oxygen glucose deprivation for the indicated times. The cell lysates were immunoblotted with the indicated antibodies. Lower panel: the graph shows the results of the quantitative analysis of the P-JNK level (n = 3). The data (a - d) are expressed as the mean ± S.E.M. of three independent experiments. Statistical comparisons were evaluated using ANOVA followed by Tukey’s HSD (a, b and d) and Dunnett’s T3 (c) post hoc analysis. ***P < 0.001, **P < 0.01, and *P < 0.05