Fig. 6

Effect of XWL-1-48 on growth of breast cancer in vivo. Athymic nude mice bearing MCF-7 tumor xenografts were orally administered every other day vehicle control (sterile normal saline), XWL-1-48 (2 and 4 mg/kg), and VP16 (23 mg/kg) for 30 days. a Relative Tumor Volume and (b) body weight were measured every other day; c The picture showed the tumor size of MCF-7 xenograft at the end of the experiment; d Tumor tissues were weighed and further calculated inhibitory rate of tumor weight. Data are mean ± SD of the tumor weight for each group of 7 experimental animals; e-i Protein expression in xenografts was determined after 30-day of XWL-1-48 or VP16 treatment. Tumors were harvested 2 h after the last dose. γ-H2AX, p-p53, p53; p-Akt, Akt, p-Mdm2 and total Mdm2 were analyzed by western blot, further quantified by image J. A representative result is shown from at least three independent experiments. *p < 0.05, **p < 0.01 vs. control, respectively