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Table 1 Proteins identified in the quantitative proteomic analysis differentially expressed across the dataset

From: Kv1.3 channel blockade with the Vm24 scorpion toxin attenuates the CD4+ effector memory T cell response to TCR stimulation

UniProt KB

Gene

Protein

Fisher’s LSD (p) US vs. OKT3

Fisher’s LSD (p) OKT3 vs. OKT3 + Vm24

Fold change OKT3/US

Fold change OKT3 + Vm24/US

Concordance with other proteomic studiesa

Q15306

IRF4

Interferon regulatory factor 4

1.4E-06

1.53E-04

13.04

6.71

1, 2

Q13283

G3BP1

Ras GTPase-activating protein-binding protein 1

7.5E-06

1.72E-05

3.90

1.31

1, 2

Q9Y3F4

STRAP

Serine-threonine kinase receptor-associated protein

8.98E-06

8.98E-06

13.95

1.00

1, 2

P14625

HSP90B1

Endoplasmin

5.94E-05

2.39E-04

2.03

1.19

1, 2

P08238

HSP90AB1

Heat shock protein HSP 90-beta

2.02E-04

1.26E-03

1.94

1.23

1, 2

P98179

RBM3

RNA-binding protein 3

1.49E-04

3.11E-04

2.43

1.15

1, 2

P35606

COPB2

Coatomer subunit beta’

8.85E-04

1.20E-03

1.90

1.04

1, 2

Q92598

HSPH1

Heat shock protein 105 kDa

5.92E-04

2.44E-02

4.26

2.61

1, 2

P53618

COPB1

Coatomer subunit beta

5.90E-04

1.05E-02

3.67

2.05

1, 2

P11142

HSPA8

Heat shock cognate 71 kDa protein

8.09E-04

4.05E-03

1.87

1.20

1, 2

P31153

MAT2A

S-adenosylmethionine synthase isoform type-2

1.47E-03

5.27E-03

7.91

2.37

1, 2

Q2YHR9

HLA-B

HLA class I histocompatibility antigen

2.08E-03

4.51E-03

2.23

1.16

1

P62995

TRA2B

Transformer-2 protein homolog beta

1.43E-03

7.12E-03

3.43

1.60

2

P11021

HSPA5

78 kDa glucose-regulated protein

3.24E-03

1.32E-02

1.62

1.14

1, 2

O43396

TXNL1

Thioredoxin-like protein 1

1.26E-03

3.45E-02

4.61

2.72

1, 2

Q14103

HNRNPD

Heterogeneous nuclear ribonucleoprotein D0

5.12E-03

1.60E-02

1.67

1.14

2

Q13435

SF3B2

Splicing factor 3B subunit 2

4.02E-03

3.16E-02

3.30

1.80

1, 2

Q15436

SEC23A

Protein transport protein Sec23A

5.22E-03

2.28E-02

3.64

1.69

2

Q07666

KHDRBS1

KH domain-containing, RNA-binding, signal transduction-associated protein 1

1E-02

8.73E-03

2.36

0.96

2

Q16629

SRSF7

Serine/arginine-rich splicing factor 7

1.28E-02

1.56E-02

2.99

1.08

2

P08195

SLC3A2

4F2 cell-surface antigen heavy chain

9.01E-05

1.88E-02

9.94

6.31

1, 2

O94925

GLS

Glutaminase kidney isoform, mitochondrial

1.59E-04

4.37E-02

6.80

4.72

2

P23381

WARS

Tryptophan--tRNA ligase, cytoplasmic

8.15E-04

4.82E-02

5.32

3.39

1, 2

O60763

USO1

General vesicular transport factor p115

1.07E-03

1.31E-01

3.15

2.42

2

P42224

STAT1

Signal transducer and activator of transcription 1-alpha/beta

2.48E-03

1.26E-01

2.98

2.20

2

P10515

DLAT

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

1.75E-03

9.08E-02

5.30

3.50

2

P23246

SFPQ

Splicing factor, proline- and glutamine-rich

3.63E-03

3.23E-01

1.59

1.44

2

O00571

DDX3X

ATP-dependent RNA helicase DDX3X

1.07E-02

2.31E-01

3.43

2.48

1, 2

P24534

EEF1B2

Elongation factor 1-beta

4.62E-02

1.9E-01

1.54

1.88

1, 2

P17980

PSMC3

26S proteasome regulatory subunit 6A

3.69E-02

9.95E-01

1.74

1.73

1, 2

P27708

CAD

CAD protein

6.06E-03

1.14E-01

1.93

1.48

1, 2

P17987

TCP1

T-complex protein 1 subunit alpha

1.98E-02

3.51E-01

2.58

2.04

1, 2

Q99613

EIF3C

Eukaryotic translation initiation factor 3 subunit C

3.55E-03

5.77E-02

3.33

2.06

1, 2

Q9UN86

G3BP2

Ras GTPase-activating protein-binding protein 2

9.56E-03

1.17E-01

3.20

2.06

1, 2

P67809

YBX1

Nuclease-sensitive element-binding protein 1

1.61E-02

7.19E-02

2.02

1.32

1, 2

P26641

EEF1G

Elongation factor 1-gamma

2.7E-02

8E-02

1.76

1.20

1, 2

P34932

HSPA4

Heat shock 70 kDa protein 4

1.93E-02

9.42E-02

2.17

1.41

1, 2

P11940

PABPC1

Polyadenylate-binding protein 1

1.15E-05

7.50E-02

2.92

2.51

1, 2

P61088

UBE2N

Ubiquitin-conjugating enzyme E2 N

4.75E-02

1.82E-03

0.50

1.48

–

P61247

RPS3A

40S ribosomal protein S3a

1.48E-03

1.65E-02

0.42

0.79

–

Q53EL6

PDCD4

Programmed cell death protein 4

1.26E-03

2.79E-02

0.26

0.67

1, 2

O00160

MYO1F

Unconventional myosin-If

6.13E-03

3.84E-02

0.52

0.84

–

Q5JSL3

DOCK11

Dedicator of cytokinesis protein 11

1.63E-03

8.98E-01

0.45

0.44

1

Q92522

H1FX

Histone H1x

1.97E-03

6.06E-02

0.46

0.72

–

P07737

PFN1

Profilin-1

3.51E-03

5.64E-01

0.47

0.55

1

O43390

HNRNPR

Heterogeneous nuclear ribonucleoprotein R

4.43E-03

1.9E-01

0.65

0.78

–

Q13561

DCTN2

Dynactin subunit 2

1.12E-02

2.14E-01

0.27

0.57

–

P16104

H2AFX

Histone H2AX

4.24E-02

9.79E-02

0.52

0.89

–

O94906

PRPF6

Pre-mRNA-processing factor 6

2.05E-02

8.37E-02

0.41

0.82

–

P62906

RPL10A

60S ribosomal protein L10a

9.61E-03

7.94E-02

0.26

0.70

–

P07766

CD3E

T-cell surface glycoprotein CD3 epsilon chain

2.02E-06

1

0.03

0.03

1

Q9UGI8

TES

Testin

9.03E-03

4.25E-01

0.66

0.74

–

Q6JBY9

RCSD1

CapZ-interacting protein

1.86E-02

2.26E-01

0.20

0.56

1

  1. Proteins with at least 1.5-fold change in either direction that were significantly (p < 0.05) different comparing the unstimulated and the OKT3-stimulated group. Concordance with other T cell activation proteomic studies is indicated. Within the OKT3-regulated proteins, comparison between the OKT3 and the OKT3 + Vm24-treated group is indicated and proteins that showed a statistically significant reduction on the amplitude of the TCR-mediated change as a result of the addition of the Vm24 toxin are indicated in bold
  2. a) Concordance with other proteomic studies:
  3. 1Tan H, Yang K, Li Y, Shaw TI, Wang Y, Blanco DB, et al. Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation. Immunity. 2017;46:488–503
  4. 2Ron-Harel N, Santos D, Ghergurovich JM, Sage PT, Reddy A, Lovitch SB, et al. Mitochondrial Biogenesis and Proteome Remodeling Promote One-Carbon Metabolism for T Cell Activation. Cell Metab. 2016;24:104–17
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