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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: TNFAIP8 promotes the proliferation and cisplatin chemoresistance of non-small cell lung cancer through MDM2/p53 pathway

Fig. 6

p53 inhibition is essential for the TNFAIP8 knockdown-mediated inhibition of NSCLC proliferation and cisplatin chemoresistance. a, b qRT-PCR and western blotting analyses showed that p53 shRNA (p53-sh) increased cyclin D1 expression levels in TNFAIP8 shRNA2 (TNFAIP8-sh2)-transfected NCI-H460 and A549 cells. *P < 0.05 and n.s., not significant (Student’s t-test.) c p53 inhibition restored NSCLC cell viability after TNFAIP8-sh2 transfection. d, e qRT-PCR and western blotting analyses showed that p53 shRNA (p53-sh) increased RAD51 expression levels in TNFAIP8 shRNA2 (TNFAIP8-sh2)-transfected A549/cDDP cells. *P < 0.05 and n.s., not significant (Student’s t-test.) f A549/cDDP cells were transfected with shRNA (Ctrl), TNFAIP8 shRNA2 (TNFAIP8-sh2) or p53 shRNA (p53-sh), and CCK-8 assay was used to measure cisplatin sensitivity. *P < 0.05 (Student’s t-test)

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