Fig. 4

TNFAIP8 knockdown enhances the chemosensitivity of NSCLC cells to cisplatin. a, b Cisplatin induced TNFAIP8 expression in a time- and dose-dependent manner. c Parental A549 and the cDDP-resistant cell line (A549/cDDP) cells were treated with the indicated doses of cisplatin. d TNFAIP8 expression was confirmed at both the transcript and protein levels in the A549 and A549/cDDP cell lines. e A549/cDDP cells transfected with control shRNA (Ctrl) or TNFAIP8 shRNAs (shRNAs) was analysed for TNFAIP8 expression by qRT-PCR (left panel) and immunoblot assay (right panel). f CCK-8 assay results showed that TNFAIP8 knockdown in both A549 and A549/cDDP cells significantly decreased the IC50. Data are expressed as the mean ± SEM (n = 3). *P < 0.05 (Student’s t-test). g TNFAIP8 KD cells transiently transfected with the empty vector as control (designated sh2/Ctrl), or a vector encoding for human TNFAIP8 to restore TNFAIP8 expression (designated sh2/R) in A549/cDDP cells. *P < 0.05 (Student’s t-test). h IC 50 of cisplatin in sh2/Ctrl or sh2/R. The IC 50 values were obtained by CCK-8 assay. Data are expressed as the mean ± SEM (n = 3). *P < 0.05 (Student’s t-test)