Fig. 5
From: Fascin induces melanoma tumorigenesis and stemness through regulating the Hippo pathway
Fascin regulatesTAZ protein stability. a Quantitative RT-PCR analysis of TAZ mRNA level in WM793 control and WM793 fascin KO cells following culture in medium without/with serum overnight. b Western blot analysis of TAZ expression in WM793 control and WM793 fascin KO cells treated with cycloheximide (CHX). c Quantification of panel B represents TAZ degradation rate in WM793 control and WM793 fascin KO cells. d Immunoblotting analysis of WM793 control and WM793 fascin KO cells after serum starvation overnight and treatment with MG132 (1 μM) for indicated times. e Western blot analysis of WM793 control and WM793 fascin KO cells following serum starvation overnight and then treatment with GSK-3β inhibitor CHIR0091 (2 μM) for 12 h