Fig. 7
From: Peptides derived from the integrin β cytoplasmic tails inhibit angiogenesis
β3-Endonexin is able to promote HUVEC tube formation but not required for VEGF-induced HUVEC adhesion and migration. a, b HUVECs were transfected with an siRNA duplex specifically targeting β3-endonexin (siβ3-endo). A non-targeting siRNA duplex (siControl) was used as a control. Cells were harvested 48 h after transfection, and expression of β3-endonexin in HUVECs was quantified by qRT-PCR for mRNA (a) and western blotting for protein (b). c, d After transfected with siRNA for 48 h, HUVECs were harvested and used for the tube formation assays. The formed capillary-like structures were counted and the disconnected structures were indicated (arrow heads). e-g HUVECs transfected with either siControl or siβ3-endo were used in adhesion (e) and migration (f, g) assays in the absence and presence of VEGF. The adhered or migrated cells were quantified as described in methods. Results were expressed as means ±SD from three experiments; statistical significance was analyzed using Student’s t test (**, p < 0.01)