Fig. 2

Constant endocytic degradation of the exon 19 deletion mutant of EGFR. a, serum-starved HCC827 cells were treated with EGF at 20 or 100 ng/ml for indicated times. Cell lysates were analyzed by immunoblottings with indicated antibodies. The graph below shows quantification data of EGFR levels. Error bars represent the standard error of the mean (n = 3). b, immunofluorescence experiments showing EGFR staining in HCC827 and H1650 cells with or without EGF treatment (20 ng/ml, 4 h). c and d, A549, HCC827, and H1650 cells were treated with 100 μM of chloroquine to block lysosomal degradation for indicated times before processed for immunofluorescence analysis to examine EGFR staining using a fluorescent microscope (Olympus BX63, 40X objective). DAPI stains the nucleus. Scale bar = 10 μm