Fig. 2

SW-13 Growth and clonogenic potential analysis. a SW-13, SW-13/V, and SW-13/S cells were plated with 50,000 cells per well and grown in complete medium (CM) for 6 days (with medium changed on day 3) and counted for viable and non-viable cells each day using 0.4% Trypan Blue. SLC12A7 overexpression had no significant effect on cell growth (two-way ANOVA) compared to their controls. Horizontal bar, mean; Error bars, standard error of mean. Cell growth analysis was performed in 2 independent experiments with 6 wells for each cell type analyzed. b SW-13, SW-13/V, and SW-13/S cells were plated at clonogenic density (5000 cells/well) and grown in CM for 6 days. Colonies with 8 or more cells were counted. SLC12A7 overexpression had no significant effect on colony growth (one-way ANOVA) compared to their controls. Horizontal bar, mean; Error bars, standard error of mean. Colony growth analysis was performed in 2 independent experiments with 6 wells for each cell type analyzed. c Representative photomicrographs of SW-13, SW-13/V, and SW-13/S cells after 6 days of colony growth