Fig. 3
From: Klotho negatively regulated aerobic glycolysis in colorectal cancer via ERK/HIF1α axis
KL negatively regulated HIF1α protein level and HIF1α transcriptional activity. HIF1α is a key factor in regulation of aerobic glycolysis. To answer whether KL regulated aerobic through HIF1α, we first examined the HIF1α protein level in KL overexpressed colon cancer cells and observed a significant decrease in HIF1α protein level (a). Then, we performed dual-luciferase assay to assess the contribution of KL on HIF1α transcriptional activity as reflected by HRE-luciferase analysis, and indicated that KL inhibited HIF1α transcriptional activity in a dose-dependent manner (b). Glycolysis is a multi-step process involved in the participation of HIF1α targeted glycolytic genes, including GLUT1, HK2, PDK1 and LDHA. In KL overexpressed colon cancer cells, the transcription level of these genes decreased (c). Western blot analysis further confirmed the role of KL in down-regulation of the protein level of these genes (d). *P < 0.05