Fig. 2
From: Klotho negatively regulated aerobic glycolysis in colorectal cancer via ERK/HIF1α axis
KL inhibited aerobic glycolysis in colon cancer cells. With the aim to assess the role of KL in glycolysis regulation in vitro, as performed a series of in vitro assay. KL overexpressed CRC cancer cells were obtained by using lentiviral mediated transfection, and the efficacy was validated by using immunoblotting with FLAG antibody (a) and quantitative PCR (b). Forced expression of KL impairs glycolysis in colon cancer cells as determined by reducing glucose consumption (c), lactate production (d), and ATP production (e).Next, the impact of KL on glycolysis rate was assessed by using Seahorse Energy Flux system through examination of ECAR, which reflects the glycolytic rate, and the result suggested that KL overexpression inhibited glycolysis. Mitochondrial respiration, reflected by OCR, usually impaired by glycolysis, and OCR results showed that OCR value increased in KL overexpressed colon cancer cells, further indicating the negative role of KL in glycolysis (f). *P < 0.05