Table 1 List of Primers used for Real Time PCR Analysis
SNo | Gene | Sequence | Annealing Temp.(°C) | Amplicon size (bp) |
|---|---|---|---|---|
1 | GAPDH | FP: 5′ - GAGAAGGCTGGGGCTCATTTGC – 3’ RP: 5′ - TGGTGCAGGAGGCATTGCTGATG – 3′ | 61 | 145 |
2 | HDAC8 | FP: 5′ - GGCTAGGTTATGATGCCCAGC – 3’ RP: 5′ - CATGATGCCACCCTCCAGACC – 3′ | 60 | 141 |
3 | HDAC6 | FP: 5′ - TGTCTCTGGAGGGTGGCTACAAC – 3’ RP: 5′ - GGAAACTGAAGCCTGGGCACTC– 3′ | 60.5 | 125 |