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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

Fig. 1

HDAC8 is predominantly cytoplasmic in localization. a Confocal images of HDAC8 localization in HeLa (upper panel) and HEK 293 T (lower panel) (Scale: 20 μm). b Nucleolar localization confirmation of HDAC8 in HeLa (Scale: 20 μm). c Confocal images of HDAC8 localization in Forskolin (10 μM) treated HeLa (upper panel) and HEK 293 T (lower panel) (Scale: 20 μm). d HDAC8 protein levels in total, cytoplasmic and nuclear fractions of HeLa and HEK 293 T. β-actin and histone-3 are loading controls. e HDAC8 enzyme activity assay with HeLa total (5 μg), cytoplasmic (5 μg) and (nuclear) 5 μg fractions in presence or absence of specific inhibitor PCI-34051(10 μM) in HeLa and HEK 293 T respectively. f HDAC8 enzyme activity assay with Forskolin (10 μM) treated, HDAC8 Immunoprecipitated fractions of total (5 μg), cytoplasmic (5 μg) and (nuclear) 5 μg fractions, along with untreated control fractions of HeLa and HEK 293 T respectively. * indicates p-value < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X