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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages

Fig. 4

SW480 and SW620-derived EV effect on immunophenotype and cytokine secretion of THP-1 monocytes (M), inactive macrophages (M0) and polarized macrophages (M1 and M2). a, b, c Flow cytometry analysis showing the percentage of CD14, HLA-DR and CD206 positive cells (mean with range, n = 4) at M, M0, M1 and M2 stages following incubation with 10 μg/mL of SW480 EVs or SW620 EVs. Statistical analysis was carried out with two-way ANOVA test. *p ≤ 0.05; **p ≤ 0.01 vs. untreated controls of the respective monocyte-macrophage cell subset. d Cytokine and chemokine secretion pattern in M, M0, M1 and M2 stages following incubation with 10 μg/mL of SW480 EVs or SW620 EVs analysed by Luminex assay (mean with range, n = 3). Statistical analysis was carried out with multiple t-tests using Holm-Sidak method for multiple comparison correction. *p ≤ 0.05 vs. untreated controls of the respective monocyte-macrophage cell subtype

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Cell Communication and Signaling

ISSN: 1478-811X