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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Interaction between human osteosarcoma and mesenchymal stem cells via an interleukin-8 signaling loop in the tumor microenvironment

Fig. 3

Effects of neutralizing anti-IL-8 Ab on IL-8 expression in mono-cultured and co-cultured MG63 and hMSCs. a Changes in IL-8 expression in MG63 were assessed by qRT-PCR. The addition of nIL-8 Ab to MG63 decreased the expression of IL-8 mRNA. b Changes in IL-8 expression in hMSCs were assessed by qRT-PCR. The addition of nIL-8 Ab to hMSCs decreased the expression of IL-8 mRNA. c Changes in IL-8 protein expression in hMSCs and MG63 were assessed by western blot analysis. The addition of nIL-8 Ab to MG63 and hMSCs decreased the expression of IL-8 protein in these cells. d The quantification of western blot analysis. Data represents represent the mean ± SD of three independent experiments. p < 0.05 was considered to indicate significance: (*) p < 0.05, (**) p < 0.01

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Cell Communication and Signaling

ISSN: 1478-811X